• 1.

  医药科学

  • 巢式pcr -引用次数：4

    Through blood screening for HCV antibodies, the infection rate of HCV in pregnant women in south of China is 1.33% (20/1506). Using nested RT-PCR, 80% (16/20) of the anti-HCV positive pregnant women are HCV RNA positive.

    通过抗-HCV进行筛查，我国南方地区孕妇人群抗-HCV阳性率为1.33％(20／1506)，这些抗—HCV阳性的孕妇采用逆转录巢式PCR方法检测HCV RNA，发现80％(16／20)呈HCV RNA阳性。

    参考来源 - 丙型肝炎病毒的垂直传播研究

  • 角蛋白20mrna -引用次数：1

    参考来源 - 结直肠癌术后早期5

• 2.

  农业科学

  • 套式rt-pcr -引用次数：8

    No cross-reactions were found in samples of infectious bovine rhinotracheitis virus,bovine influenza virus type 3,and bovine viral diarrhea virus. These results indicated that the nested RT-PCR was specific for BRSV detection.

    用该套式RT-PCR对牛传染性鼻气管炎病毒、牛副流感病毒3型、牛病毒性腹泻病毒等进行了检测,结果无交叉反应,表明该检测方法具有良好的特异性。

    参考来源 - 套式RT

  • 巢式rt-pcr -引用次数：8

    Evaluation of Four BTV detection systems to BTV-5The nucleic acid real-time fluorescent RT-PCR detection system, nucleic acid RT-PCR detection system, OIE standard nested RT-PCR detection method and antigen-captured ELISA detection system were evaluated through specificity tests and sensitiveness tests.

    二、四种BTV检测体系对BTV-5全病毒检测的评价对蓝舌病病病毒核酸实时荧光RT-PCR检测体系、核酸RT-PCR检测体系、OIE标准巢式RT-PCR检测方法和抗原捕获ELISA检测体系进行特异性和灵敏度评价。

    参考来源 - 蓝舌病病毒四种检测技术比较研究

  • 套式逆转录聚合酶链扩增法

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